Liquid nitrogen vapor phase is a storage method used to preserve biological samples, including cells, tissues, and biological molecules, at ultra-low temperatures (-150°C to -196°C). This method involves storing the samples in a container filled with liquid nitrogen, where they are exposed to the vapor phase above the liquid nitrogen.
In this method, the samples are typically first placed in a vial or tube that is designed to withstand low temperatures and then placed into a cryobox. The cryobox is then lowered into a larger container filled with liquid nitrogen, so that the samples are in the vapor phase above the liquid nitrogen. This phase provides a stable and consistent temperature for long-term storage of biological samples, which helps to maintain their viability and integrity.
This storage method is a popular option for long-term preservation of biological samples, especially for those that are valuable or irreplaceable. Compared to other methods of low-temperature storage, such as freezers, this phase storage offers a lower risk of cross-contamination, as well as a lower risk of freezer failures or power outages. However, it is important to follow proper safety protocols when handling liquid nitrogen, as it can be dangerous if mishandled.