Frozen cell vials are containers used to store and preserve cells for long-term storage at ultra-low temperatures, typically below -80°C. Frozen cell vials can be used for various applications, such as cell banking, long-term storage of valuable or rare cell lines, or for experimental replicates.
To prepare frozen cell vials, cells are typically grown to a suitable density, trypsinized or detached using a dissociation agent, and then resuspended in a freezing medium containing a cryoprotectant, such as DMSO or glycerol. The cell suspension is then aliquoted into sterile vials, usually at a concentration of 1-2 million cells per mL, and then frozen rapidly in a controlled-rate freezer or a -80°C freezer.
The use of cryoprotectants is essential to prevent damage to the cells during the freezing process. Cryoprotectants act by reducing the formation of ice crystals, which can damage cell membranes and structures, and by stabilizing proteins and other cellular components. The optimal concentration and type of cryoprotectant can vary depending on the cell type and the specific application.
Frozen cell vials should be stored in a dedicated freezer, typically at -80°C or below, to ensure long-term viability and stability. Proper labeling and tracking of the vials are also important to ensure easy retrieval and identification of specific cell lines or samples.
When needed, frozen cell vials can be thawed by rapidly warming the vial in a 37°C water bath or incubator, and then transferring the cell suspension to a culture dish or flask containing fresh medium. It is important to optimize the thawing and recovery process for each cell line and application to ensure optimal viability and recovery.