CHO-DXB11 is a cell line derived from Chinese hamster ovary (CHO) cells. It was generated by transfecting CHO-K1 cells with the gene for the dihydrofolate reductase (DHFR) enzyme, which is involved in the synthesis of nucleic acids. The transfected cells were then selected for their ability to grow in a medium containing methotrexate, a drug that inhibits DHFR activity.
The resulting CHO-DXB11 cell line is a DHFR-deficient cell line that requires the addition of exogenous folic acid and thymidine to the growth medium for survival. This property allows for the selection of cells that have incorporated and are expressing a foreign gene that confers DHFR activity and resistance to methotrexate. Therefore, CHO-DXB11 cells are commonly used in the production of recombinant proteins, as they allow for the selection of stable cell lines that have integrated and are expressing the gene of interest.
CHO-DXB11 cells have been used to produce a wide range of proteins, including monoclonal antibodies, enzymes, and growth factors. They are particularly useful in biomanufacturing, as they allow for the production of large quantities of recombinant proteins with high purity and consistency.
Overall, the generation of CHO-DXB11 has been an important development in biomanufacturing, as it has allowed for the efficient and consistent production of a wide range of therapeutic proteins and other biologics.